Coding

Part:BBa_K4891007:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)


aroL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 425
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 425
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These recombinant plasmids pBAD33-aroL serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.

References